Regulatory

Part:BBa_K945001:Design

Designed by: Daniel Villalobos Ramírez   Group: iGEM12_Tec-Monterrey_EKAM   (2012-09-26)


FLD1 yeast promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
    Illegal XbaI site found at 797
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
    Illegal XbaI site found at 797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 25
    Illegal EcoRI site found at 342
    Illegal EcoRI site found at 675
    Illegal XbaI site found at 797
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter poses an assembly problem by having two EcoRI restriction sites in its sequence, which, if modified, would potentially alter its function in initiation of transcription.


Source

This sequence was identified from the 5' untranslated region of the glutathione-dependent formaldehyde dehydrogenase (FLD1) gene found in Pichia pastoris.

References